Elucidation of acute toxicity of drugs by real-time metabolome analysis and multivariate time-series analysis
Description: In this study, our research team have developed a real-time metabolome analytical technique using probe electrospray ionization tandem mass spectrometry (PESI/MS/MS) with time-series multivariate analyses. We optimized analytical conditions for real-time mass spectrometry, achieving successfully monitoring of 40 metabolites in a living mouse. Our research team has also developed a data pipeline using the R software, and this data pipeline can automatically execute bioinformatics analyses such as multivariate analysis. Moreover, we have developed a new approach using a state space model based on Bayesian statistical modeling for time-series data obtained from real-time monitoring. Finally, we applied this technique to a living mouse, demonstrating the practicality of our technique.
Amount: ¥4,420,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2018-04-01
End date: 2021-03-31
Investigator: Kei Zaitsu
Institution: Nagoya University
Funded outputs count: 9
Funder award ID: 18K10122
OpenAlex ID: G1960078460