Glycolysis, but not Mitochondria, responsible for intracellular ATP distribution in cortical area of podocytes
Description: Differentiated podocytes maintain glomerular physiology and require substantial levels of energy. Mitochondria and glycolysis are two major producers of ATP, but the precise roles of each in podocytes remain unknown. Mitochondria in differentiated podocytes are present in the central part of cell body. However, blocking mitochondria had minor effects on cell shape and migratory ability. In contrast, blocking glycolysis significantly reduced the formation of lamellipodia, a cortical area of these cells, and decreased cell migratory ability. Intracellular ATP distribution in lamellipodia was regulated only by glycolysis. Inhibiting glycolysis resulted in apoptosis, prevented differentiation, and impaired lamellipodia formation while inhibition of mitochondria reduced synaptopodin expression. These data suggest that mitochondria and glycolysis play parallel but distinct roles in differentiated and differentiating podocytes.
Amount: ¥4,940,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2014-04-01
End date: 2017-03-31
Investigator: Takahiko Nakagawa (lead)
Institutions: Kyoto University, Nara Medical University, Nara Medical University(2016)Kyoto University(2014-2015)
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Funded outputs count: 2
Funder award ID: 26460274
OpenAlex ID: G3383175841