Analysis of the transcription factor network during the early stage of iPSC production
Description: Genome-wide expression analyses were used to identify transcription factors whose expression decrease at the early stage or reprogramming. They were further narrowed down by other data base to select for the genes that are likely to be regulated directly by Oct4 and Klf4. The selection resulted in 38 candidate genes whose expression changes are potentially important for the early stage of reprogramming. siRNA-mediated knockdown and over-expression of these genes showed that down-regulation of Osr1、Ebf1、Ebf3、Meox2、Smardc3 and Prrx is essential at the early stage of reprogramming. The involvement of these genes in EMT or MET was examined by the used of NMuMG cells, and the Ebf3 was found to have an activity in promoting EMT.
Amount: ¥5,200,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2013-04-01
End date: 2016-03-31
Investigator: Koji Hisatake
Institution: University of Tsukuba
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 10
Funder award ID: 25460354
OpenAlex ID: G4189959930