Synthetic DNA-free circuits for “scarless” programming of mammalian cells
Description: Relevance to Public Health This proposal aims to develop a new platform for the construction and delivery of protein-level circuits that program the behavior of mammalian cells while avoiding mutagenizing the host genome. This platform will facilitate diverse biomedical applications wherever it is necessary to eliminate or fix pathological cells, or generate new ones on demand in human patients. Specific examples include a “smart” virus that senses intracellular hallmarks and lyses cancer cells, and one that responds to different stages of cell fate reprogramming and dynamically and efficiently ushers cells through this process in vivo.
Funding type: career_development
Funder scheme: K99
Start date: 2019-04-01
End date: 2020-03-31
Investigator: XIAOJING J GAO
Institution: CALIFORNIA INSTITUTE OF TECHNOLOGY
Funded outputs count: 2
Funder award ID: K99EB027723
OpenAlex ID: G4240297672