Regulatory mechanism in metabolic allocation of nitrogen-containing compounds from central metabolisms to specialized metabolisms in plants
Description: Most of plant alkaloids are produced from amino acids. In this study, the molecular basis of metabolite allocation from amino acid metabolism (central metabolism) to alkaloid biosynthesis (specialized metabolism) was investigated. Quinolizidine alkaloids (QA) and lycopodium alkaloids (LA) are derived from lysine. is lysine decarboxylation. The cDNAs encoding lysine/ornithine decarboxylase (L/ODC) catalyzing the first step QA and LA biosynthesis were isolated from several species and characterized using recombinant protein expressed in E. coli. The enzymes from QA/LA-producing plants exhibited relatively higher lysine-decarboxylase activities comparing to those of other species. It is suggested that this difference leads the change in metabolic allocation from lysine metabolism to alkaloid biosynthesis.
Amount: ¥5,590,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2012-04-01
End date: 2015-03-31
Investigator: Mami YAMAZAKI (lead)
Institution: Chiba University
Funded outputs count: 5
Funder award ID: 24570041
OpenAlex ID: G4787627271