Interdisciplinary research to accelerate making the Japanese fire-bellied newt a model animal: groundwork for technology and information and its practical application.
Description: To install the resource, technology and information for the study of Japanese fire-bellied newt into various research areas, 1) we generated various lines of newts that were created by transgenesis or genome editing on demand, and established systems for cell tracking and conditional gene regulation by means of grafting of tissues from transgenic newts or by using cell-specific promoters. We successfully applied these systems to various research areas including the regenerative physiology. 2) We constructed a transcriptome database ‘IMORI’and opened it to the public (http://antler.is.utsunomiya-u.ac.jp/imori/). On the basis of sequence information in the database, molecular cloning and gene expression analyses were facilitated in various research areas. 3) We made it possible that the newt is reared from egg to juvenile (after metamorphosis) efficiently in Imori-no-Sato, the newt stock center in the field. We started to supply larvae and juveniles for research purpose.
Amount: ¥47,320,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (A)
Start date: 2012-05-31
End date: 2016-03-31
Investigator: Chikafumi CHIBA (lead)
Institution: University of Tsukuba
Subfield: Molecular Biology
Domain: Life Sciences
Funded outputs count: 21
Funder award ID: 24240062
OpenAlex ID: G497745856