Development of new breeding material using dominant mutants causing by insertions of DNA transposon, nDart1
Description: Transposons occupying a large portion of the rice genome. Active DNA transposons are important tools for gene functional analysis. The endogenous non-autonomous transposon, nDart1, in rice is said to generate various transposon-insertion mutants because nDart1 elements tend to insert into genic regions under natural growth conditions.The Lgg mutant which was isolated from nDart1-promoted mutant plants bore slightly large grains as a dominant inheritance. Transposon-display identified the insertion site of nDart1 in the Lgg mutant. Identified LGG genes show the similarity with RNA binding proteins. The expression of LGG gene in Lgg mutant was lower than WT. Lgg mutation was essentially caused by the insertion of nDart1, knock-out mutant using genome editing (GE) and over-expressing (OE) lines were generated. GE lines showed large grain phenotypes that were the same as Lgg mutants. On the contrary, harvested seeds from the OE line were smaller than the WT and GE lines.
Amount: ¥4,810,000
Funding type: research
Funder scheme: Grant-in-Aid for Scientific Research (C)
Start date: 2016-04-01
End date: 2020-03-31
Investigator: Kazuo Tsugane (lead)
Institution: National Institute for Basic Biology
Subfield: Genetics
Domain: Life Sciences
Funded outputs count: 5
Funder award ID: 16K07561
OpenAlex ID: G5407932184