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Significance of Corneal Cell Invasion by Bacteria

Description: Project Narrative: P. aeruginosa is a leading cause of blinding corneal infections that are also difficult to treat using currently available therapeutics. We have found that when these bacteria enter corneal epithelial cells, they traffic to multiple destinations wherein they express unique virulence and survival factors. Here, we will explore the significance of this intracellular diversification to the bacteria, to the host cell, and to infection in vivo, with the goal of better understanding disease pathogenesis and best practices for its management.
Amount: $401,300
Funding type: research
Funder scheme: R01
Start date: 1995-07-01
End date: 2026-06-30
Investigator: SUZANNE MJ FLEISZIG
Institution: UNIVERSITY OF CALIFORNIA BERKELEY
Subfield: Ophthalmology
Field: Medicine
Funded outputs count: 79
Funder award ID: 5r01ey011221-26
OpenAlex ID: G6991847692
Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis
2025 · Daniel Schator, Naren G. Kumar, et al. · Nature Communications · 7
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The Pseudomonas aeruginosa T3SS can contribute to traversal of an in situ epithelial multilayer independently of the T3SS needle
2025 · Eric Jedel, Daniel Schator, et al. · mBio · 2
Contact Lens Wear Alters Transcriptional Responses to Pseudomonas aeruginosa in Both the Corneal Epithelium and the Bacteria
2025 · Naren G. Kumar, Melinda R Grosser, et al. · Investigative Ophthalmology & Visual Science · 1
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Intracellular replication of Pseudomonas aeruginosa in epithelial cells requires suppression of the caspase-4 inflammasome
2023 · Abby R Kroken, Keith A Klein, et al. · mSphere · 18
Pseudomonas aeruginosa Can Diversify after Host Cell Invasion to Establish Multiple Intracellular Niches
2022 · Naren G. Kumar, Vincent Nieto, et al. · mBio · 43
Exotoxin S secreted by internalized Pseudomonas aeruginosa delays lytic host cell death
2022 · Abby R. Kroken, Naren Gajenthra Kumar, et al. · PLoS Pathogens · 37
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Nerve‐associated transient receptor potential ion channels can contribute to intrinsic resistance to bacterial adhesion in vivo
2021 · Stephanie J. Wan, Ananya Datta, et al. · The FASEB Journal · 14
Resistance of the murine cornea to bacterial colonization during experimental dry eye
2020 · Stephanie J. Wan, Sophia Ma, et al. · PLoS ONE · 16
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Contact lens-related corneal infection: Intrinsic resistance and its compromise
2019 · Suzanne M.J. Fleiszig, Abby R. Kroken, et al. · Progress in Retinal and Eye Research · 142
Type IV Pili Can Mediate Bacterial Motility within Epithelial Cells
2019 · Vincent Nieto, Abby R. Kroken, et al. · mBio · 37
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Impact of topical corticosteroid pretreatment on susceptibility of the injured murine cornea to Pseudomonas aeruginosa colonization and infection
2018 · Yvonne T. Wu, Tan N. Truong, et al. · Experimental Eye Research · 11
IL-1R and MyD88 Contribute to the Absence of a Bacterial Microbiome on the Healthy Murine Cornea
2018 · Stephanie J. Wan, Aaron B. Sullivan, et al. · Frontiers in Microbiology · 28
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The Impact of ExoS on Pseudomonas aeruginosa Internalization by Epithelial Cells Is Independent of fleQ and Correlates with Bistability of Type Three Secretion System Gene Expression
2018 · Abby R. Kroken, Camille K. Chen, et al. · mBio · 57
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Epithelial cell lysates induce ExoS expression and secretion by Pseudomonas aeruginosa
2018 · Victoria Hritonenko, Matteo Metruccio, et al. · FEMS Microbiology Letters · 7
Quantification of Bacterial Twitching Motility in Dense Colonies Using Transmitted Light Microscopy and Computational Image Analysis
2018 · Benjamin Smith, Jianfang Li, et al. · BIO-PROTOCOL · 2
Corneal surface glycosylation is modulated by IL‐1R and Pseudomonas aeruginosa challenge but is insufficient for inhibiting bacterial binding
2017 · Amber L. Jolly, Paresh Agarwal, et al. · The FASEB Journal · 10
Human Tear Fluid Reduces Culturability of Contact Lens-Associated Pseudomonas aeruginosa Biofilms but Induces Expression of the Virulence-Associated Type III Secretion System
2016 · Yvonne T. Wu, Connie Tam, et al. · The Ocular Surface · 11
Pseudomonas aeruginosa Outer Membrane Vesicles Triggered by Human Mucosal Fluid and Lysozyme Can Prime Host Tissue Surfaces for Bacterial Adhesion
2016 · Matteo M. E. Metruccio, David J. Evans, et al. · Frontiers in Microbiology · 61
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Pseudomonas aeruginosa Survival at Posterior Contact Lens Surfaces after Daily Wear
2015 · Yvonne T. Wu, Lucia S. Zhu, et al. · Optometry and Vision Science · 19
Pseudomonas aeruginosa-Induced Bleb-Niche Formation in Epithelial Cells Is Independent of Actinomyosin Contraction and Enhanced by Loss of Cystic Fibrosis Transmembrane-Conductance Regulator Osmoregulatory Function
2015 · Amber L. Jolly, Desire Takawira, et al. · mBio · 43
The Importance of the Pseudomonas aeruginosa Type III Secretion System in Epithelium Traversal Depends upon Conditions of Host Susceptibility
2015 · Aaron B. Sullivan, K. P. Connie Tam, et al. · Infection and Immunity · 30
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Cytotoxic clinical isolates of Pseudomonas aeruginosaidentified during the Steroids for Corneal Ulcers Trial show elevated resistance to fluoroquinolones
2014 · Durga S Borkar, Nisha R Acharya, et al. · BMC Ophthalmology · 31
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Cornea and Ocular Surface Disease
2014 · Danielle M. Robertson, Larry J. Alexander, et al. · Optometry and Vision Science · 3
Surfactant Protein D Contributes to Ocular Defense against Pseudomonas aeruginosa in a Murine Model of Dry Eye Disease
2013 · Susan R. Heimer, David J. Evans, et al. · PLoS ONE · 13
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Why Does the Healthy Cornea Resist Pseudomonas aeruginosa Infection?
2013 · David J. Evans, Suzanne M.J. Fleiszig · American Journal of Ophthalmology · 84
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