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Dynamics of Antimicrobial Peptide Interactions with Bacterial Membranes

Description: PUBLIC HEALTH RELEVANCE: Especially in hospital settings, bacteria are increasingly resistant to drugs. Antimicrobial peptides are the front-line defense against pathogens throughout the animal kingdom, but we do not really understand how they work to kill bacterial cells. This work develops novel imaging methods that enable direct observation of the mechanisms of bacterial killing by antimicrobials with better spatial and time resolution than ever before.
Funding type: research
Funder scheme: R01
Start date: 2010-08-01
End date: 2019-11-30
Investigator: JAMES C. WEISSHAAR
Institution: UNIVERSITY OF WISCONSIN-MADISON
Subfield: Microbiology
Funded outputs count: 32
Funder award ID: R01GM094510
OpenAlex ID: G7948752355
Antimicrobial peptoids pass rapidly through bacterial membranes and flocculate ribosomes and DNA: A single-cell fluorescence study
2026 · Yanyu Zhu, Josefine Eilsø Nielsen, et al. · Proceedings of the National Academy of Sciences · 1
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Real-Time Fluorescence Microscopy on Living E. coli Sheds New Light on the Antibacterial Effects of the King Penguin β-Defensin AvBD103b
2022 · Céline Landon, Yanyu Zhu, et al. · International Journal of Molecular Sciences · 7
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Local rigidification and possible coacervation of the Escherichia coli DNA by cationic nylon-3 polymers
2021 · Yanyu Zhu, Lei Liu, et al. · Biophysical Journal · 8
Long-term effects of the proline-rich antimicrobial peptide Oncocin112 on the Escherichia coli translation machinery
2020 · Yanyu Zhu, James C. Weisshaar, et al. · Journal of Biological Chemistry · 21
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Biophysical Properties of Escherichia coli Cytoplasm in Stationary Phase by Superresolution Fluorescence Microscopy
2020 · Yanyu Zhu, Mainak Mustafi, et al. · mBio · 40
Resistance of early stationary phase E. coli to membrane permeabilization by the antimicrobial peptide Cecropin A
2019 · Anurag Agrawal, Nambirajan Rangarajan, et al. · Biochimica et Biophysica Acta (BBA) - Biomembranes · 32
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Near Saturation of Ribosomal L7/L12 Binding Sites with Ternary Complexes in Slowly Growing E. coli
2019 · Mainak Mustafi, James C. Weisshaar · Journal of Molecular Biology · 4
Faculty Opinions recommendation of Rigidification of the Escherichia coli cytoplasm by the human antimicrobial peptide LL-37 revealed by superresolution fluorescence microscopy.
2019 · Harold Erickson · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
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Rigidification of the Escherichia coli cytoplasm by the human antimicrobial peptide LL-37 revealed by superresolution fluorescence microscopy
2018 · Yanyu Zhu, Sonisilpa Mohapatra, et al. · Proceedings of the National Academy of Sciences · 94
Single‐Cell, Time‐Lapse Reactive Oxygen Species Detection in E. coli
2018 · Zhilin Yang, Heejun Choi · Current Protocols in Cell Biology · 12
HaloTag Assay Suggests Common Mechanism of E. coli Membrane Permeabilization Induced by Cationic Peptides
2018 · Zhilin Yang, James C. Weisshaar · ACS Chemical Biology · 20
Effects of alterations of the E. coli lipopolysaccharide layer on membrane permeabilization events induced by Cecropin A
2018 · Anurag Agrawal, James C. Weisshaar · Biochimica et Biophysica Acta (BBA) - Biomembranes · 30
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Simultaneous Binding of Multiple EF-Tu Copies to Translating Ribosomes in Live Escherichia coli
2018 · Mainak Mustafi, James C. Weisshaar · mBio · 33
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Melittin-Induced Permeabilization, Re-sealing, and Re-permeabilization of E. coli Membranes
2018 · Zhilin Yang, Heejun Choi, et al. · Biophysical Journal · 90
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Oxidative stress induced in E. coli by the human antimicrobial peptide LL-37
2017 · Heejun Choi, Zhilin Yang, et al. · PLoS Pathogens · 79
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Spatial Distribution and Ribosome-Binding Dynamics of EF-P in Live Escherichia coli
2017 · Sonisilpa Mohapatra, Heejun Choi, et al. · mBio · 32
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Single-cell, time-resolved study of the effects of the antimicrobial peptide alamethicin on Bacillus subtilis
2016 · Kenneth J. Barns, James C. Weisshaar · Biochimica et Biophysica Acta (BBA) - Biomembranes · 27
Lights, Camera, Action! Antimicrobial Peptide Mechanisms Imaged in Space and Time
2015 · Heejun Choi, Nambirajan Rangarajan, et al. · Trends in Microbiology · 81
Single-Cell, Time-Resolved Antimicrobial Effects of a Highly Cationic, Random Nylon-3 Copolymer on Live Escherichia coli
2015 · Heejun Choi, Saswata Chakraborty, et al. · ACS Chemical Biology · 48
Effects of amino acid starvation on RelA diffusive behavior in live E scherichia coli
2015 · Wenting Li, Emmanuelle Bouveret, et al. · Molecular Microbiology · 33
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The spatial biology of transcription and translation in rapidly growing Escherichia coli
2015 · Somenath Bakshi, Heejun Choi, et al. · Frontiers in Microbiology · 128
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Single-cell, real-time detection of oxidative stress induced in Escherichia coli by the antimicrobial peptide CM15
2015 · Heejun Choi, Zhilin Yang, et al. · Proceedings of the National Academy of Sciences · 147
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Time‐dependent effects of transcription‐ and translation‐halting drugs on the spatial distributions of the E scherichia coli chromosome and ribosomes
2014 · Somenath Bakshi, Heejun Choi, et al. · Molecular Microbiology · 135
Medium Effects on Minimum Inhibitory Concentrations of Nylon-3 Polymers against E. coli
2014 · Heejun Choi, Saswata Chakraborty, et al. · PLoS ONE · 41
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Nonperturbative Imaging of Nucleoid Morphology in Live Bacterial Cells during an Antimicrobial Peptide Attack
2014 · Somenath Bakshi, Heejun Choi, et al. · Applied and Environmental Microbiology · 58
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