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Elucidation of the laminin recognition mechanism by integrin

Description: Recognition of laminin, a major component of the basement membrane, by integrin receptors plays a central role in the adhesion of epithelial cells to basement membrane, but the structural background of this molecular interaction has been unclear. In this study, we determined the three-dimensional structure of the laminin-integrin complex and elucidated its molecular recognition mechanism.By using the previously developed antibody fragment, Fv-clasp, we succeeded in preparing a stable laminin-integrin complex sample and determined its structure at 3.9 Å-resolution by single particle cryo-electron microscopy. In addition, binding analysis using mutants of amino acid residues found at the binding interface revealed by the complex structure quantitatively clarified the contribution of each amino acid residue to the binding.

Funder: Japan Society for the Promotion of Science

Amount: ¥10,790,000

Funding type: research

Funder scheme: Grant-in-Aid for Scientific Research (B)

Start date: 2018-04-01

End date: 2021-03-31

Investigator: Takao Arimori

Institution: Osaka University

Topic: Cell Adhesion Molecules Research

Subfield: Immunology and Allergy

Field: Medicine

Domain: Health Sciences