Analysis of molecular dynamics in neuronal dendrites by simultaneous multi-point fluorescence correlation spectroscopy
Description: This research project aimed to measure dynamics of protein molecules rapidly moving in cytosole of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz time of resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII protein was expressed, and in analyzing by FCS.
Amount: ¥3,500,000
Funding type: research
Funder scheme: Grant-in-Aid for Challenging Exploratory Research
Investigator: Takafumi INOUE
Institution: Waseda University
Funded outputs count: 7
Funder award ID: 22650066
OpenAlex ID: G877323948