Keyword

directed evolution

Description: A protein or nucleic acid engineering method that uses iterative variation and selection to develop molecules with desired properties.

Primary topic: Enzyme Catalysis and Immobilization (33%)

Wikidata ID: wiki/Q440681

OpenAlex ID: keywords/directed-evolution

Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

2004 · Nathan C Shaner, Robert E Campbell, et al. · Nature Biotechnology · 4,615

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

2017 · Nicole M. Gaudelli, Alexis C. Komor, et al. · Nature · 4,169

Efficient proximity labeling in living cells and organisms with TurboID

2018 · Tess C Branon, Justin A Bosch, et al. · Nature Biotechnology · 2,144

PDF

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

2010 · Christopher K. Savile, Jacob M. Janey, et al. · Science · 1,760

Synthesizing life

2001 · Jack W. Szostak, David P. Bartel, et al. · Nature · 1,680

Bacterial alkaline proteases: molecular approaches and industrial applications

2002 · Gupta R., Beg Q., et al. · Applied Microbiology and Biotechnology · 1,603

Programming cells by multiplex genome engineering and accelerated evolution

2009 · Harris H. Wang, Farren J. Isaacs, et al. · Nature · 1,527

Industrial enzyme applications

2002 · Ole Kirk, Torben Vedel Borchert, et al. · Current Opinion in Biotechnology · 1,465

Directed evolution of APEX2 for electron microscopy and proximity labeling

2014 · Stephanie S Lam, Jeffrey D Martell, et al. · Nature Methods · 1,382

PDF

Horseradish peroxidase: a modern view of a classic enzyme

2004 · Nigel C. Veitch · Phytochemistry · 1,380

Lipases for biotechnology

2002 · Karl-Erich Jaeger, Thorsten Eggert · Current Opinion in Biotechnology · 1,379

Outlook for cellulase improvement: Screening and selection strategies

2006 · Y.-H. Percival Zhang, Michael E. Himmel, et al. · Biotechnology Advances · 1,349

Kemp elimination catalysts by computational enzyme design

2008 · Daniela Röthlisberger, Olga Khersonsky, et al. · Nature · 1,308

A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons

2016 · D. Gowanlock R. Tervo, Bum-Yeol Hwang, et al. · Neuron · 1,182

PDF

Bacterial lipases: an overview of production, purification and biochemical properties

2004 · R. Gupta, N. Gupta, et al. · Applied Microbiology and Biotechnology · 1,178

Exploring protein fitness landscapes by directed evolution

2009 · Philip A. Romero, Frances H. Arnold · Nature Reviews Molecular Cell Biology · 1,166

PDF

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

2010 · Jeremy J. Agresti, Eugene Antipov, et al. · Proceedings of the National Academy of Sciences · 1,154

Directed Evolution: Bringing New Chemistry to Life

2017 · Frances H. Arnold · Angewandte Chemie International Edition · 1,148

PDF

RNA-peptide fusions for the in vitro selection of peptides and proteins

1997 · Richard W. Roberts, Jack W. Szostak · Proceedings of the National Academy of Sciences · 1,107

Bacterial Biocatalysts: Molecular Biology, Three-Dimensional Structures, and Biotechnological Applications of Lipases

1999 · K-E. Jaeger, B. W. Dijkstra, et al. · Annual Review of Microbiology · 1,091

Machine-learning-guided directed evolution for protein engineering

2019 · Kevin K. Yang, Zachary Wu, et al. · Nature Methods · 1,085

PDF

Synthetic biology: new engineering rules for an emerging discipline

2006 · Ernesto Andrianantoandro, Subhayu Basu, et al. · Molecular Systems Biology · 1,080

A transposase strategy for creating libraries of circularly permuted proteins

2012 · Manan M. Mehta, Shirley Liu, et al. · Nucleic Acids Research · 1,042

Methods for the directed evolution of proteins

2015 · Michael S. Packer, David R. Liu · Nature Reviews Genetics · 996

Selecting and screening recombinant antibody libraries

2005 · Hennie R Hoogenboom · Nature Biotechnology · 973

Works count: 10,100

Citation count: 300,300