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Keyword

gene expression normalization

Description: Methods adjust gene expression measurements against suitable reference genes or other controls to enable reliable comparisons.
OpenAlex ID: keywords/gene-expression-normalization
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
2001 · Kenneth J. Livak, Thomas D. Schmittgen · Methods · 180,496
limma powers differential expression analyses for RNA-sequencing and microarray studies
2015 · Matthew E. Ritchie, Belinda Phipson, et al. · Nucleic Acids Research · 40,245
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A new mathematical model for relative quantification in real-time RT-PCR
2001 · M. W. Pfaffl · Nucleic Acids Research · 34,555
Adjusting batch effects in microarray expression data using empirical Bayes methods
2006 · W. Evan Johnson, Cheng Li, et al. · Biostatistics · 8,621
A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples.
1993 · P Chomczynski · PubMed · 2,788
Singular value decomposition for genome-wide expression data processing and modeling
2000 · Orly Alter, Patrick O. Brown, et al. · Proceedings of the National Academy of Sciences · 1,971
Real-time RT-PCR normalisation; strategies and considerations
2005 · J Huggett, K Dheda, et al. · Genes and Immunity · 1,858
Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
2006 · Nicholas Silver, Steve Best, et al. · BMC Molecular Biology · 1,727
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An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis.
2013 · Xiayu Rao, Xuelin Huang, et al. · PubMed · 1,550
Housekeeping genes as internal standards: use and limits
1999 · O. Thellin, W. Zorzi, et al. · Journal of Biotechnology · 1,545
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STATISTICAL METHODS FOR IDENTIFYING DIFFERENTIALLY EXPRESSED GENES IN REPLICATED cDNA MICROARRAY EXPERIMENTS
2002 · Sandrine Dudoit, Jean Yang, et al. · 1,401
Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress
2005 · Nathalie Nicot, Jean-François Hausman, et al. · Journal of Experimental Botany · 1,332
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Human housekeeping genes, revisited
2013 · Eli Eisenberg, Erez Y. Levanon · Trends in Genetics · 1,283
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Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR
2006 · Mukesh Jain, Aashima Nijhawan, et al. · Biochemical and Biophysical Research Communications · 1,185
An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development
2006 · Karen E Reid, Niclas Olsson, et al. · BMC Plant Biology · 885
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Control Selection for RNA Quantitation
2000 · Toshihide Suzuki, Paul J. Higgins, et al. · BioTechniques · 876
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Q-Gene: processing quantitative real-time RT–PCR data
2003 · Perikles Simon · Bioinformatics · 858
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Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process
2008 · Marino Expósito-Rodríguez, Andrés A Borges, et al. · BMC Plant Biology · 791
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A method for the absolute quantification of cDNA using real-time PCR
2003 · Joseph A Whelan, Nick B Russell, et al. · Journal of Immunological Methods · 784
Evidence Based Selection of Housekeeping Genes
2007 · Hendrik J. M. de Jonge, Rudolf S. N. Fehrmann, et al. · PLoS ONE · 748
A general and flexible method for signal extraction from single-cell RNA-seq data
2018 · Davide Risso, Fanny Perraudeau, et al. · Nature Communications · 717
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The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription‐polymerase chain reaction (RT‐PCR) analysis in plants
2008 · Laurent Gutierrez, Mélanie Mauriat, et al. · Plant Biotechnology Journal · 696
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Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
2009 · Anna R Paolacci, Oronzo A Tanzarella, et al. · BMC Molecular Biology · 691
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Selection of reliable reference genes for gene expression studies in peach using real-time PCR
2009 · Zhaoguo Tong, Zhihong Gao, et al. · BMC Molecular Biology · 674
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The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
2005 · K. Dheda, J.F. Huggett, et al. · Analytical Biochemistry · 668
Works count: 4,827
Citation count: 387,600
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