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Keyword

PCR optimization

Description: PCR optimization is the adjustment of polymerase chain reaction conditions to improve the reliability, efficiency, and specificity of DNA amplification.
Wikidata ID: wiki/Q7226557
OpenAlex ID: keywords/pcr-optimization
‘Touchdown’ PCR to circumvent spurious priming during gene amplification
1991 · R.H. Don, P. T. Cox, et al. · Nucleic Acids Research · 2,587
Inhibition and facilitation of nucleic acid amplification
1997 · I G Wilson · Applied and Environmental Microbiology · 2,266
Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
1993 · Russell Higuchi, Carita Fockler, et al. · Nature Biotechnology · 2,163
High-Coverage ITS Primers for the DNA-Based Identification of Ascomycetes and Basidiomycetes in Environmental Samples
2012 · Hirokazu Toju, Akifumi S. Tanabe, et al. · PLoS ONE · 1,256
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Environmental DNA
2018 · Pierre Taberlet, Aurélie Bonin, et al. · 1,062
PCR protocols — A guide to methods and applications
1990 · H SELIGER · Trends in biotechnology · 1,062
Multiplex PCR: Optimization and Application in Diagnostic Virology
2000 · Elfath M. Elnifro, Ahmed M. Ashshi, et al. · Clinical Microbiology Reviews · 962
Multiplex PCR: Critical Parameters and Step-by-Step Protocol
1997 · O. Henegariu, N.A. Heerema, et al. · BioTechniques · 954
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology
2008 · Cindy J. Smith, A. Mark Osborn · FEMS Microbiology Ecology · 937
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Multiplex polymerase chain reaction: A practical approach
2002 · P. Markoulatos, N. Siafakas, et al. · Journal of Clinical Laboratory Analysis · 647
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OPTIMIZATION OF PCRs
1990 · Michael A. Innis, David H. Gelfand · Elsevier eBooks · 643
Effective amplification of long targets from cloned inserts and human genomic DNA.
1994 · S Cheng, C Fockler, et al. · Proceedings of the National Academy of Sciences · 626
Touchdown PCR for increased specificity and sensitivity in PCR amplification
2008 · Darren J Korbie, John S Mattick · Nature Protocols · 625
Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development
2018 · Tao Wang, Changying Chen, et al. · Biotechnology Advances · 528
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Betaine Improves the PCR Amplification of GC-Rich DNA Sequences
1997 · W. Henke, K. Herdel, et al. · Nucleic Acids Research · 510
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Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
2012 · Todd C. Lorenz · Journal of Visualized Experiments · 492
PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR
1996 · S. Ahn · Nucleic Acids Research · 474
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The use of random amplified polymorphic DNA markers in wheat
1992 · K. M. Devos, M. D. Gale · Theoretical and Applied Genetics · 423
Multiplex PCR: advantages, development, and applications.
1994 · M C Edwards, R A Gibbs · Genome Research · 416
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High and Low Annealing Temperatures Increase Both Specificity and Yield in Touchdown and Stepdown PCR
1996 · Karl H. Hecker, Kenneth H. Roux · BioTechniques · 412
Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR
2009 · Clare E. Holleley, Paul G. Geerts · BioTechniques · 376
Revolutions in Rapid Amplification of cDNA Ends: New Strategies for Polymerase Chain Reaction Cloning of Full-Length cDNA Ends
1995 · B.C. Schaefer · Analytical Biochemistry · 321
Formamide can dramatically improve the specificity of PCR
1990 · Gobinda Sarkar, Stephen Kapelner, et al. · Nucleic Acids Research · 305
Microarray-based DNA methylation profiling: technology and applications
2006 · A. Schumacher · Nucleic Acids Research · 294
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Systematic Design of 18S rRNA Gene Primers for Determining Eukaryotic Diversity in Microbial Consortia
2014 · Luisa W. Hugerth, Emilie E. L. Muller, et al. · PLoS ONE · 281
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Works count: 3,750
Citation count: 42,740
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