A Histochemical Study of Some Post-Fertilization Developmental Stages in Dipcadi montanum Dalz
Year: 1976
Type: article
Abstract: The stages in the seed development of Dipcadi montanum are studied using cytochemical staining techniques for DNA, polysaccharides, RNA and proteins. The embryo development conforms to the Chenopodiad type and that of the endosperm follows the Helobial pattern. The zygote nucleus is azur A-positive and the nuclei of the embryo cells in successive developmental stages are also equally azur A positive. However, the nuclei in the cells of the old embryo in the mature seed respond faintly for azur A. The cytoplasm in the zygote and the cells of the embryo during successive developmental stages is PAS-positive. The cells of the cotyledon derived from l tier and partially from l' tier show the accumulation of the starch grains in the mature seed. The suspensor formed by the derivatives of cb possesses rich accumulation of proteins and starch grains in the young proembryo stages. Cytoplasmic RNA and proteins are generally rich in all the cells of the proembryo except in suspensor in which RNA is invariably low and proteins are occassionally rich.In the endosperm, the chalazal chamber is metabolically more active than the micropylar chamber. The nuclei per unit area are more in the cytoplasm of the chalazal chamber than in the cytoplasm of the micropylar chamber. The chalazal chamber has higher cytoplasmic RNA, proteins and PAS-positiveness than the micropylar chamber. The nuclei in the chalazal chamber often fuse among themselves to form hypertrophied ones in the maturing seed. These nuclei are densely azur A positive but exhibit distortion in shape.The persistent antipodal cells are metabolically very active. They are rich in cytoplasmic polysaccharides, RNA and proteins. Their nuclei are very much enlarged and stain densely with azur A.The seed coat becomes black in the mature seed.
Source: CYTOLOGIA
Authors: J. Syamasundar, M. G. Panchaksharappa
Institution: Karnatak University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W1006896554