PRODUCCIÓN Y EFECTO ANTAGÓNICO DE QUITINASAS Y GLUCANASAS POR Trichoderma spp., EN LA INHIBICIÓN DE Fusarium subglutinans y Fusarium oxysporum IN VITRO
Year: 2005
Type: article
Abstract: Hydrolytic enzymes, chitinases and glucanases, produced by Trichoderma spp strains were quantified, and their in vitro antibiotic effect on the growth of the mycelium and on the reproductive potential of Fusarium subglutinans and F. oxysporum was assessed. The production of chitinases oscillated between 0.33 and 2.29 mmol of N-acetilglucosamine, and that of glucanases oscillated between 14.4 to 60.6 mmol of glucose. The T. harzianum (Thzcf-9 and Thzcf-6) chitinases inhibited the growth of the mycelium of F. oxysporum in 34 and 27 % and of F. subglutinans 35 and 21%, respectively. The reproductive potential in the production of conidia decreased from 95 and 86 %, while their viability varied 66 and 73 % in F. oxysporum and F. subglutinans, respectively. The T. koningii (Tkcf-2) and T. harzianum (Thzcf-6) glucanases inhibited the growth of the mycelium of F. oxysporum 44 and 32 % and in the case of F. subglutinans 38 and 34 %, respectively. It was also possible to reduce conidia production of both species of Fusarium by 95 % and viability in F. oxysporum and F. subglutinans by 74 and 64 %, respectively. The results obtained under laboratory conditions suggest that T. harzianum (Thzcf-9 and Thzcf-6) for quitinase activity, as well as T. koningii (Tkcf-2) and T. harzianum (Thzcf-6) for glucanases activity have potential as biocontrol agents on the two species of Fusarium involved in the etiology of mango malformation.
Authors: Centro de Estudios Profesionales del Colegio Superior Agropecuario del Estado de Guerrero, A.C. Michel-Aceves, M.A. Otero-Sánchez, O. Rebolledo-Domínguez, R. Lezama-Gutiérrez, M.E. Ochoa-Moreno
Language: Spanish
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $200
OpenAlex ID: W1489965337