Work

The bacteriophage P22 arc and mnt repressors. Overproduction, purification, and properties.

Year: 1985

Type: article

Abstract: The arc and mnt genes of bacteriophage P22 encode small repressor proteins. We have cloned these genes onto plasmids that overproduce Arc and Mnt to greater than 1% of the soluble cellular protein. Both proteins were purified to greater than 95% homogeneity, and N-terminal sequences and amino acid compositions were determined. These data, in combination with previously determined gene sequences, establish the complete protein sequences for Arc (53 residues) and Mnt (82 residues). Both proteins have melting temperatures between 45 and 55 degrees C and can be renatured to a fully active species. Arc is a dimer in solution and Mnt is a tetramer.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(17)38995-0

Authors: A K Vershon, P Youderian, M M Susskind, R T Sauer

Institutions: University of Southern California, University of Massachusetts Chan Medical School, Massachusetts Institute of Technology

Language: English

Topic: Bacteriophages and microbial interactions

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funders: National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases