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A novel method for the fluorometric assay of proteins using hypochlorite-thiamine reagent.

Year: 1980

Type: article

Abstract: A fluorometric assay method for proteins based on the reaction of peptide groups is described. The N-H groups of peptides are chlorinated with sodium hypochlorite, and the resulting N-chloropeptides are allowed to react with thiamine, giving fluorescent thiochrome. The present method is highly sensitive and is applicable to 1-10μg of protein. Little variability of fluorescence intensity was observed among proteins.

Source: Chemical and Pharmaceutical Bulletin

DOI: 10.1248/cpb.28.641

Authors: TOSHIO KINOSHITA, JUNICHIRO MURAYAMA, KAZUYO MURAYAMA, AKIO TSUJI

Institutions: SHOWA Medical University, YUKI Gosei Kogyo Co., Ltd. (Japan), Kitasato University

Language: English

Topic: Analytical Methods in Pharmaceuticals

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: bronze

OpenAlex ID: W1528295787

FWCI: 0.717

Cites:

Cited by: 5

Related to: 10