Work

Molecular cloning of the complete genome of strain GDVII of Theiler's virus and production of infectious transcripts

Year: 1989

Type: article

Abstract: We constructed a complete cDNA clone of strain GDVII of Theiler's virus in Bluescript plasmid. This recombinant plasmid, called pTMGDVII, was used to synthesize full-length RNA transcripts of the viral insert. This RNA was infectious for BHK cells. Virus R1-GDVII, obtained from transfected BHK cells, caused rapidly fatal encephalomyelitis in BALB/c mice. High amounts of viral antigens were present in neurons. No antigens were found in white matter. Therefore, the phenotype of R1-GDVII was indistinguishable from that of genuine GDVII Theiler's virus.

Source: Journal of Virology

DOI: 10.1128/jvi.63.3.1101-1106.1989

Authors: F Tangy, A McAllister, M Brahic

Institutions: Centre National de la Recherche Scientifique, Institut Pasteur

Language: English

Topic: Multiple Sclerosis Research Studies

Subfield: Clinical Neurology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W1536714839

FWCI: 3.822

Cites: 16

Cited by: 37

Related to: 10