Work

Capacity for Steroidogenesis of Adrenals in Hypophysectomized and Adrenocorticotropic Hormone-treated Hypophysectomized Rats

Year: 1973

Type: article

Abstract: The steroidogenic capacity, determined by in vitro superfusion with adrenocorticotropic hormone (ACTH), of adrenals in hypophysectomized rats decays with a half-life of approximately 6 hours. In vivo ACTH treatment of rats within 12 hours posthypophysectomy leads to regeneration of this capacity with little or no lag period. In vivo ACTH treatment of rats 24 hours posthypophysectomy leads to regeneration of this capacity with a lag period of 3 to 6 hours, indicating some unknown changes in the adrenals between 12 and 24 hours posthypophysectomy. With 36 hours posthypophysectomized rats, no regeneration of this capacity was observed for 24 hours of in vivo ACTH treatment. The regenerative effect of ACTH is blocked by actinomycin D but not by puromycin, indicating that an RNA factor is involved. It is postulated that this RNA is, or includes, the mRNA coding for the steroidogenic protein(s) reported by Garren and co-workers ((1965) Proc. Nat. Acad. Sci. U.S.A. 53, 1443).

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)43405-4

Authors: M.K. Mostafapour, T.T. Tchen

Institution: Wayne State University

Language: English

Topic: Adrenal Hormones and Disorders

Subfield: Endocrinology, Diabetes and Metabolism

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430