Year: 2015
Type: article
Abstract: Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells with consumable costs of $0.10-$0.20 per cell and includes five lanes for simultaneous experiments. We anticipate that this system will serve as a general platform for single-cell imaging and sequencing.
Source: Genome biology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,490
Funders: David and Lucile Packard Foundation, Howard Hughes Medical Institute, Columbia University, National Institutes of Health, Stand Up To Cancer, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering, National Center for Chronic Disease Prevention and Health Promotion, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Common Fund
Awards: G158795805, G3854734828, Single cell mapping of developmental trajectories underlying health and disease, Topology of Cancer Evolution and Heterogeneity, A Microfluidic System for Image-Guided RNA-Seq of Single Cells in Glioblastoma, Multiplexed, Single Molecule Protein Identification for Single Cell Proteomics
OpenAlex ID: W1668035141