Nuclear DNA content in some plant kinds used as an external standard in flow cytometry
Year: 2014
Type: article
Abstract: The aim of research was to determine DNA content and genome size of certain plant varieties for subsequent use as an external standard in flow cytometry of plants. The main challenge is to find widespread kinds of cultivated plants, allowing exploring most of the plant objects with DNA content in the range from 0.1 to 60 pg. Furthermore, the data from international databases were analyzed and compared with the actual DNA content of the home (Russian) kinds of plants. Recommendations for the optimization of the experimental methods and data interpretation in flow cytometry are also provided. Several plant kinds are recognized as most suitable for the use as an external standard. Kinds of Lepidium, Sinapis, Petroselinum, Pisum, Lathyrus, and Vicia reveal good parameters of nuclei isolation, high amount of isolated nuclei, low amount of destroyed nuclei, and thus low background fluorescence, which is important for the experiment.
Source: Turczaninowia
Authors: M.V. Skaptsov, S.V. Smirnov, M.G. Kutsev
Institution: Altai State University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W1709416643