Year: 1989
Type: article
Abstract: Immunological studies have shown that plants contain intermediate-filament antigens, but it is not known whether these proteins are capable in themselves of forming filaments. To address this problem, a detergent-resistant and high-salt-insoluble fraction from carrot (Daucus carota L.) suspension cells was solubilized with 9 M-urea and then subjected to a two-step dialysis procedure, devised for the reconstitution of animal intermediate filaments. This induced the self-assembly of 10 nm filaments and large bundles of filaments. The predominant components of reconstituted material were polypeptides with apparent molecular masses between 58 and 62 kDa. These polypeptides immunoblotted with two monoclonal antibodies known to show broad cross-reactivity with intermediate filaments across the phylogenetic spectrum. This establishes that the antigens are able to self-assemble into intermediate-sized filaments.
Source: Biochemical Journal
DOI: 10.1042/bj2610679
Authors: A J Hargreaves, K C Goodbody, C W Lloyd
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: bronze
Funder: Royal Society
OpenAlex ID: W175012045