Work

Role of Krüppel-like factor 5 in the maintenance of the stem cell niche in the intestinal crypt

Year: 2015

Type: article

Abstract: deletion specifically from the Lgr5-expressing CBC cells in adult mouse intestine using an inducible Cre recombinase system. Shortly (3-5 days) after Cre induction, proliferation of both CBC and TA cells ceased, which was accompanied by an increase in apoptosis in the crypt. Beginning at two weeks following Cre induction, both Klf5 expression and proliferation re-appeared but without the re-emergence of Lgr5-positive CBC cells, which were eventually depleted by four months following induction. These findings indicate that KLF5 plays an important role in regulating proliferation and survival of CBC stem cells in the intestine.

Source: Stem Cell and Translational Investigation

DOI: 10.14800/scti.839

Authors: Jes G Kuruvilla, Amr M Ghaleb, Agnieszka B Bialkowska, Mandayam O Nandan, Vincent W Yang

Institutions: Stony Brook School, Stony Brook Medicine, Stony Brook University

Language: English

Topic: Kruppel-like factors research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases