High‐density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool
Year: 2015
Type: article
Abstract: In wheat, a lack of genetic diversity between breeding lines has been recognized as a significant block to future yield increases. Species belonging to bread wheat's secondary and tertiary gene pools harbour a much greater level of genetic variability, and are an important source of genes to broaden its genetic base. Introgression of novel genes from progenitors and related species has been widely employed to improve the agronomic characteristics of hexaploid wheat, but this approach has been hampered by a lack of markers that can be used to track introduced chromosome segments. Here, we describe the identification of a large number of single nucleotide polymorphisms that can be used to genotype hexaploid wheat and to identify and track introgressions from a variety of sources. We have validated these markers using an ultra-high-density Axiom(®) genotyping array to characterize a range of diploid, tetraploid and hexaploid wheat accessions and wheat relatives. To facilitate the use of these, both the markers and the associated sequence and genotype information have been made available through an interactive web site.
Source: Plant Biotechnology Journal
DOI: 10.1111/pbi.12485
Authors: Mark O. Winfield, Alexandra M. Allen, Amanda J. Burridge, Gary L. A. Barker, Harriet R. Benbow, Paul A. Wilkinson, Jane Coghill, Christy Waterfall, Alessandro Davassi, Geoff Scopes, Ali Pirani, Teresa Webster, Fiona Brew, Claire Bloor, Julie King, Claire West, Simon Griffiths, Ian King, Alison R. Bentley, Keith J. Edwards
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
Estimated APC paid: $3,719
Awards: Enhancing diversity in UK wheat through a public sector prebreeding programme, BrisSynBio: Bristol Centre for Synthetic Biology, Releasing natural variation in bread wheat by modulating meiotic crossovers, Pre-Breeding at NIAB - Ppd alleles and markers QTL for earliness per se and novel variation from synthetic wheat useful to UK/EU wheat improvement, CerealsDB: A community resource for wheat genomics, Exploitation of interspecific biodiversity for wheat improvement, Enhancing diversity in UK wheat through a public sector prebreeding programme, 15-IWYP -Wider and faster: high-throughout phenotypic exploration of novel genetic variation for breeding high biomass and yield in wheat, Exploitation of related species for developing superior adapted wheat varieties, Purchase of Affymetrix GeneTitan for use in high throughput genotyping, G6327480397, Indo-UK Centre for the improvement of Nitrogen use Efficiency in Wheat (INEW), Development and validation of a flexible genotyping platform for wheat, Enhancing diversity in UK wheat through a public sector prebreeding programme
OpenAlex ID: W1925156307