Identification, evolution, and expression partitioning of miRNAs in allopolyploidBrassica napus
Year: 2015
Type: article
Abstract: The recently published genome of Brassica napus offers for the first time the opportunity to gain insights into the genomic organization and the evolution of miRNAs in oilseed rape. In this study, 12 small RNA libraries from two B. napus cultivars (Tapidor and Ningyou7) and their four double-haploid lines were sequenced, employing the newly sequenced B. napus genome, together with genomes of its progenitors Brassica rapa and Brassica oleracea. A total of 645 miRNAs including 280 conserved and 365 novel miRNAs were identified. Comparative analysis revealed a high level of genomic conservation of MIRNAs (75.9%) between the subgenomes of B. napus and its two progenitors' genomes, and MIRNA lost/gain events (133) occurred in B. napus after its speciation. Furthermore, significant partitioning of miRNA expressions between the two subgenomes in B. napus was detected. The data of degradome sequencing, miRNA-mediated cleavage, and expression analyses support specific interactions between miRNAs and their targets in the modulation of diverse physiological processes in roots and leaves, as well as in biosynthesis of, for example, glucosinolates and lipids in oilseed rape. These data provide a first genome-wide view on the origin, evolution, and genomic organization of B. napus MIRNAs.
Source: Journal of Experimental Botany
DOI: 10.1093/jxb/erv420
Institutions: Zhejiang University, Huazhong Agricultural University, Christian-Albrechts-Universität zu Kiel, Commonwealth Scientific and Industrial Research Organisation, Centre National de la Recherche Scientifique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Institut National de la Recherche Agronomique, Université d'Évry Val-d'Essonne, University of York
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
Estimated APC paid: $4,341
Funders: Deutscher Akademischer Austauschdienst, National Natural Science Foundation of China, China Scholarship Council, Jiangsu Collaborative Innovation Center for Modern Crop Production, Biotechnology and Biological Sciences Research Council, National Key Research and Development Program of China
OpenAlex ID: W1930564483