Work

Tyrosine Phosphorylation of the Proto‐oncogene Product Vav and Its Association with the Adapter Grb2/Ash in a Human Leukemia Cell Line UT‐7

Year: 1995

Type: article

Abstract: The vav proto-oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte-macrophage colony-stimulating factor, interleukin-3, and erythropoietin induce tyrosine phosphorylation of Vav in a human leukemia cell line UT-7. Tyrosine phosphorylation of Vav is rapid and transient; it occurs within 1 min of the stimulation and at physiological concentrations of the factors. Furthermore, we show that Vav is constitutively associated with the adapter molecule Grb2/Ash in UT-7. These data suggest that tyrosine kinases, the adapter Grb2/Ash, and the guanine nucleotide exchange factor Vav are members of a signaling pathway leading to Ras activation in hematopoietic cells.

Source: Japanese Journal of Cancer Research

DOI: 10.1111/j.1349-7006.1995.tb03061.x

Authors: Yutaka Hanazono, Ko Sasaki, Hideharu Odai, Toshihide Mimura, Kinuko Mitani, Yoshio Yazaki, Hisamaru Hirai

Institution: The University of Tokyo

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W1964549183

FWCI: 0.519

Cites: 43

Cited by: 6

Related to: 10