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In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level

Year: 2005

Type: article

Abstract: The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3A' reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutants is similar to the wild-type strain. The results do not support the longstanding hypothesis that IF1 could perform discriminatory functions while blocking the aminoacyl-tRNA acceptor site (A-site) of the ribosome. One cold-sensitive IF1 mutant shows a general overexpression, in particular at low temperatures, of both reporter genes at the protein but not mRNA level.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2004.12.072

Authors: Victor V. Croitoru, Margarete Bucheli-Witschel, Leif A. Isaksson

Institution: Stockholm University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Vetenskapsrådet

OpenAlex ID: W1965178458

FWCI: 0.516

Cites: 38

Cited by: 15

Related to: 10