Work

Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs

Year: 2008

Type: article

Abstract: Cationic amino acid transporter b 0,+ AT (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition.In the present study, we describe the cloning and sequencing of porcine b 0,+ AT.Based on the sequence of porcine b 0,+ AT deposited in the NCBI (National Center for Biotechnological Information), we identified a putative porcine homologue.Using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine b 0,+ AT was isolated.The porcine b 0,+ AT cDNA was 1,680 bp long, encoding a 487 amino acid trans-membrane protein.The predicted amino acid sequence was found to have 88.9% and 87.1% identity with human and mouse b 0,+ AT, respectively.Real-time RT-PCR indicated porcine b 0,+ AT transcripts expressed in heart, kidney, muscle and small intestine.The small intestine had the highest b 0,+ AT mRNA abundance while the muscle had the lowest (p<0.05).Along the longitudinal axis, the ileum had the highest b 0,+ AT mRNA abundance while the colon had the lowest (p<0.05).The b 0,+ AT mRNA level was highest on day 7 and 90 in the duodenum (p<0.05).It increased from day 1 to day 26 in the jejunum (p>0.05) and had the highest abundance on day 60 (p<0.05).There was, however, no difference between day 1, 7, 26, 30, 90 and 150 (p>0.05).The strongest b 0,+ AT expression appeared on day 7 in the ileum before weaning, and then decreased till day 30 but rose gradually again from day 60 to 150 (p<0.05).(

Source: Asian-Australasian Journal of Animal Sciences

DOI: 10.5713/ajas.2008.80044