Work

Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

Year: 2008

Type: article

Abstract: Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3+/-0.4 ms were achieved with the single-layer microfluidic device.

Source: Optics Letters

DOI: 10.1364/ol.33.001887

Authors: Tom Robinson, Prashant Valluri, Hugh B. Manning, Dylan M. Owen, Ian Munro, Clifford B. Talbot, Christopher Dunsby, John F. Eccleston, Geoff S. Baldwin, Mark A. A. Neil, Andrew J. de Mello, Paul M. W. French

Institutions: Imperial College London, National Institute for Medical Research

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

Funder: Engineering and Physical Sciences Research Council

OpenAlex ID: W1966682803

FWCI: 1.978

Cites: 12

Cited by: 28

Related to: 10