26S rDNA-Based Phylogenetic Investigation of Japanese Cattle-Associated Prototheca zopfii Isolates
Year: 2010
Type: article
Abstract: The study has been conducted to assess the applicability of partial 26S ribosomal DNA sequence for the phylogenic analysis of P.zopfii and to arrive relationships between specific genotype of P.zopfii and clinical mastitis. The phylogenetic analysis indicated that all genotype 2 isolates were grouped into the cluster of type strain of P.zopfii. Thus, all mastitis isolates in Japan were identified as P.zopfii genotype 2 and were independent of the cluster of the genotype 1 isolates. In one clinical case, a fecal isolate could not be identified by the 18S rDNA-based genotype specific PCR-assay and appeared to belong to a same cluster with the type strain of P. blaschkeae by 26S rDNA analysis. The isolate was the first isolation in Japan.
DOI: 10.1292/jvms.09-0115
Authors: Yuji KISHIMOTO, Rui KANO, Haruhiko MARUYAMA, Masanobu ONOZAKI, Koichi MAKIMURA, Takaaki ITO, Kiyoshi MATSUBARA, Atsuhiko HASEGAWA, Hiroshi KAMATA
Institutions: Nihon University, Kanto Chemical (Japan), Teikyo University
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W1968189189