Genetic Diversity and Transmission Characteristics of Beijing Family Strains of Mycobacterium tuberculosis in Peru
Year: 2012
Type: article
Abstract: Beijing family strains of Mycobacterium tuberculosis have attracted worldwide attention because of their wide geographical distribution and global emergence. Peru, which has a historical relationship with East Asia, is considered to be a hotspot for Beijing family strains in South America. We aimed to unveil the genetic diversity and transmission characteristics of the Beijing strains in Peru. A total of 200 Beijing family strains were identified from 2140 M. tuberculosis isolates obtained in Lima, Peru, between December 2008 and January 2010. Of them, 198 strains were classified into sublineages, on the basis of 10 sets of single nucleotide polymorphisms (SNPs). They were also subjected to variable number tandem-repeat (VNTR) typing using an international standard set of 15 loci (15-MIRU-VNTR) plus 9 additional loci optimized for Beijing strains. An additional 70 Beijing family strains, isolated between 1999 and 2006 in Lima, were also analyzed in order to make a longitudinal comparison. The Beijing family was the third largest spoligotyping clade in Peru. Its population structure, by SNP typing, was characterized by a high frequency of Sequence Type 10 (ST10), which belongs to a modern subfamily of Beijing strains (178/198, 89.9%). Twelve strains belonged to the ancient subfamily (ST3 [n=3], ST25 [n=1], ST19 [n=8]). Overall, the polymorphic information content for each of the 24 loci values was low. The 24 loci VNTR showed a high clustering rate (80.3%) and a high recent transmission index (RTI(n-1)=0.707). These strongly suggest the active and on-going transmission of Beijing family strains in the survey area. Notably, 1 VNTR genotype was found to account for 43.9% of the strains. Comparisons with data from East Asia suggested the genotype emerged as a uniquely endemic clone in Peru. A longitudinal comparison revealed the genotype was present in Lima by 1999.
Source: PLoS ONE
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,382
Funders: Institute for Fermentation, Osaka, Wellcome Trust, Ministry of Education, Culture, Sports, Science and Technology, Ministry of Health, Labour and Welfare, National Institutes of Health, Medical Research Council, Japan Society for the Promotion of Science, Fogarty International Center, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: Can we get new insights into epidemiology of tuberculosis through whole genome sequencing analysis ?, G2172969756, Community randomised evaluation of socioeconomic intervention to prevent TB, 高速旋光測定装置によるロドプシンの構造変化の動的研究, G4187727168, G5280095593, Optimizing the translation of MODS into policy and practice to maximize utility and impact, Fogarty International Clinical Research Scholars Support Center @ Vanderbilt-AAMC, Novel techniques for the detection of Pediatric TB in Ethiopia, Global Disease Research Training Program in Peru, G9000315650
OpenAlex ID: W1968329618