Work

CD169 + macrophages are sufficient for priming of CTLs with specificities left out by cross-priming dendritic cells

Year: 2015

Type: article

Abstract: Dendritic cells (DCs) are considered the most potent antigen-presenting cells (APCs), which directly prime or cross-prime MHC I-restricted cytotoxic T cells (CTLs). However, recent evidence suggests the existence of other, as-yet unidentified APCs also able to prime T cells. To identify those APCs, we used adenoviral (rAd) vectors, which do not infect DCs but selectively accumulate in CD169(+) macrophages (MPs). In mice that lack DCs, infection of CD169(+) MPs was sufficient to prime CTLs specific for all epitopes tested. In contrast, CTL responses relying exclusively on cross-presenting DCs were biased to selected strong MHC I-binding peptides only. When both DCs and MPs were absent, no CTL responses could be elicited. Therefore, CD169(+) MPs can be considered APCs that significantly contribute to CTL responses.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1423356112

Authors: Caroline A. Bernhard, Christine Ried, Stefan Kochanek, Thomas Brocker

Institutions: Ludwig-Maximilians-Universität München, Universität Ulm

Language: English

Topic: Immunotherapy and Immune Responses

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: Deutsche Forschungsgemeinschaft