Comprehensive characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies
Year: 2013
Type: article
Abstract: Missense mutations in LRRK2 (leucine-rich repeat kinase 2) are a major cause of PD (Parkinson's disease). Several antibodies against LRRK2 have been developed, but results using these polyclonal antibodies have varied widely leading to conflicting conclusions. To address this challenge, the Michael J. Fox Foundation for Parkinson's Research generated a number of monoclonal antibodies targeting epitopes across the LRRK2 protein. In the present paper, we report optimized protocols and results for ten monoclonal antibodies for immunoblotting, immunohistochemistry, immunoprecipitation and kinase activity assays, in rat, mouse and human brain tissue. Several efficacious antibodies were identified, but results demonstrate that the mouse monoclonal N241A/34 is suitable for most applications, with the best overall rabbit monoclonal antibody being c41-2. These antibodies produced a dominant band of the expected size via immunoblotting and a lack of labelling in tissue derived from LRRK2-knockout animals under optimized conditions. A significant proportion of LRRK2 protein localizes to insoluble fractions and no evidence of truncated LRRK2 protein was detected in any fraction from rodent or human tissues. An assay was developed for the robust detection of LRRK2 kinase activity directly from frozen mouse and human brain tissue, but precipitous declines in activity were observed that corresponded to increasing post-mortem intervals and processing times. Finally, we demonstrate the highest levels of brain-localized LRRK2 in the striatum, but note differential expression patterns between rat and mouse in both striatum and cortex. Anti-LRRK2 monoclonal antibodies that are unlimited in availability together with the proposed standardized protocols should aid in the definition of LRRK2 function in both health and disease.
Source: Biochemical Journal
DOI: 10.1042/bj20121742
Institutions: University of Dundee, MRC Protein Phosphorylation and Ubiquitylation Unit, Mayo Clinic in Florida, University of Alabama at Birmingham, Icahn School of Medicine at Mount Sinai, Banner Sun Health Research Institute, The University of Texas Southwestern Medical Center, Michael J. Fox Foundation
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Funders: Michael J. Fox Foundation for Parkinson's Research, Arizona Department of Health Services, University of Texas Southwestern Medical Center, Wellcome Trust, Arizona Biomedical Research Commission, Medical Research Council, National Institute on Aging, National Institute of Mental Health, National Institute of Neurological Disorders and Stroke, National Institute of Child Health and Human Development
Awards: G1052080286, G1576660248, G1606131442, G1783170366, G2161113364, G250893170, Neurobiology of Alzheimer's Disease and Aging, G2898431357, Functional Characterization of LRRK2 Knock-out and Knock-in Mouse Models, A systematic investigation into the pathogenesis and course of Parkinson's syndrome, G3250808129, G3251040009, G3521153818, G3903239027, Synapse Targeting and Assembly, G4791902628, Role of Sema7A in functional organization of neocortex, G6030318873, Arizona Alzheimer's Disease Core Center, G7161884846, National Brain and Tissue Resource for Parkinson's Disease and Related Disorders, G7747731141, G7836467104, G7887587830, G837617365, A systematic investigation into the pathogenesis and course of Parkinson's syndrome., Mechanisms of LRRK2 Mediated Neurotoxicity, Training Program in Neuroscience, G961669115
OpenAlex ID: W1969997447