Year: 2014
Type: article
Abstract: We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.
Source: FEBS Letters
Institutions: Beijing University of Technology, Beijing Proteome Research Center, State Key Laboratory of Proteomics, East China Normal University, Zhejiang University, The University of Texas Health Science Center at Houston, Model Animal Research Center, Nanjing University, Baylor College of Medicine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W1970358564