Work

Assays for Porphyrins, δ-Aminolevulinic-Acid Dehydratase, and Porphyrinogen Synthetase in Microliter Samples of Whole Blood: Applications to Metabolic Defects Involving the Heme Pathway

Year: 1972

Type: article

Abstract: Three micromethods are described for the assay of enzymes or products of the heme biosynthetic pathway in blood. A fluorometric method for the assay of protoporphyrin may be applied to the rapid screening of children for chronic lead poisoning. A colorimetric assay for delta-aminolevulinic-acid dehydratase may be applied to the detection of acute and chronic lead poisoning. A fluorometric assay for porphyrin formation from prophobilinogen is also described.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.69.9.2381

Authors: S. Granick, S. Sassa, J. L. Granick, R. D. Levere, A. Kappas

Institutions: Rockefeller University, State University of New York, SUNY Downstate Health Sciences University

Language: English

Topic: Porphyrin Metabolism and Disorders

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, U.S. Public Health Service, NIH Clinical Center