Work

Studies on the Pathogenesis of the Ethanol-induced Fatty Liver. I. Synthesis and Oxidation of Fatty Acids by the Liver*

Year: 1961

Type: article

Abstract: The present report is a study of changes in hepatic fatty acid synthesis and oxidation following ethanol administration in vivo and in vitro.Observations on the role of reduced and oxidized diphosphopyridine nucleotides are also presented.A subsequent report will deal with studies on lipid transport. MATERIALS AND METHODSPalmitate-1-C1' (specific activity 5 X 10' cpm per j.mole) was obtained from Volk Radiochemical Co., Chicago, Ill., and purified as described previously (8).Acetate- 1-C"' was purchased from New England Nuclear Corporation, Boston, Mass.Diphosphopyridine nucleotide (DPN) was obtained from Sigma Chemical Co., St. Louis, Mo.Hyamine-1OX was prepared according to the method of Passman, as modified by Eisenberg (9).Female albino rats (Charles River Laboratories, Boston, Mass.), weighing between 180 and 250 g and maintained on Purina Chow were used throughout this investigation.

Source: Journal of Clinical Investigation

DOI: 10.1172/jci104366

Authors: Gilberto Rebouças, Kurt J. Isselbacher

Institutions: Harvard University, Massachusetts General Hospital

Language: English

Topic: Alcohol Consumption and Health Effects

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: W.K. Kellogg Foundation, National Institutes of Health