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A comparative study on the catalytic properties of guanyl‐specific ribonucleases

Year: 1992

Type: article

Abstract: The kinetic parameters of reactions catalyzed by four guanyl-specific RNases T1, Pb1, Th1 and Sa were studied comparatively using three types of substrates; guanosine-2',3'-cyclophosphates, GpN dinucleoside phosphates and synthetic polyribonucleotides. The kinetic parameters were shown to be similar in spite of considerable differences in primary structures of these RNases, including amino acid residues of the active sites. Therefore, primary structures of guanyl RNases allow for a considerable number of substitutions (both in the 'recognising' and catalytical parts of the active site) without changes in the catalytical parameters.

Source: European Journal of Biochemistry

DOI: 10.1111/j.1432-1033.1992.tb16622.x

Authors: Gennady I. YAKOVLEV, Gennady P. MOISEYEV, Svetlana I. BEZBORODOVA, Valentin BOTH, Jozef SEVCIK

Institutions: Engelhardt Institute of Molecular Biology, Academy of Sciences of the Soviet Union, Genetika, Slovak Academy of Sciences, Institute of Molecular Biology of the Slovak Academy of Sciences

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W1981197890

FWCI: 0.671

Cites: 18

Cited by: 22

Related to: 10