Work

Effect of Moderate UVC Irradiation on Bovine Serum Albumin and Complex with Antimetabolite 5-Fluorouracil: Fluorescence Spectroscopic and Molecular Modelling Studies

Year: 2015

Type: article

Abstract: The interaction of antimetabolite 5-fluorouracil (5FU) with bovine serum albumin (BSA) under UVC (253.7 nm) irradiation was investigated in the present study using UV-Vis spectroscopy, steady state/time resolved fluorescence spectroscopic techniques. The stability of protein was found to be very strong when BSA gets bind to 5FU and moreover it is compared with the free BSA under UVC irradiation. From the fluorescence spectroscopic study, the stability of the complex was found to acquire 2-fold stronger than free protein. From the molecular modelling studies, we came to know the hydrogen bonds between BSA and antimetabolite 5FU are strong, up to 70.4 J/m2under UVC irradiation.

Source: International Journal of Spectroscopy

DOI: 10.1155/2015/315764

Authors: Shanmugavel Chinnathambi, Subramani Karthikeyan, Devadasan Velmurugan, Nobutaka Hanagata, Prakasarao Aruna, Singaravelu Ganesan

Institutions: National Institute for Materials Science, Anna University, Chennai, University of Madras, Hokkaido University

Language: English

Topic: Protein Interaction Studies and Fluorescence Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Department of Atomic Energy, Government of India, Board of Research in Nuclear Sciences

Awards: G3504901761, G5440021229, G7584337094