Work

Deletion of the Gly600 Residue of Alicyclobacillus acidocaldarius Squalene Cyclase Alters the Substrate Specificity into that of the Eukaryotic‐Type Cyclase Specific to (3S)‐2,3‐Oxidosqualene

Year: 2004

Type: article

Abstract: Removal service: A deletion mutant lacking the Gly600 residue of a prokaryotic squalene–hopene cyclase was prepared. Surprisingly, the mutant cyclase has no enzyme activity for 1 and 3, but shows a high conversion ratio for 2 (see scheme). Deleting Gly600 alters the specificity from prokaryotic into that of eukaryotic-type cyclases.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.200461523

Authors: Tsutomu Hoshino, Kunio Shimizu, Tsutomu Sato

Institution: Niigata University

Language: English

Topic: Plant biochemistry and biosynthesis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W1983418110

FWCI: 1.366

Cites: 33

Cited by: 41

Related to: 10