Year: 2003
Type: article
Abstract: Dextran modified with the mono-6-pentylene-diamino-6-deoxy-beta-cyclodextrin derivative was evaluated as a thermoprotectant additive for trypsin. The optimum temperature for trypsin activity was increased by 7 degrees C in the presence of this polymer. The enzyme thermostability was increased from 48.5 to 64 degrees C over 10 min of incubation, and the activation free energy of thermoinactivation at 50 degrees C was increased by 4.1 kJ/mol in the presence of the additive. Trypsin was 6-fold more resistant to autolytic inactivation at alkaline pH in the presence of the polymer.
Source: Biotechnology and Bioengineering
DOI: 10.1002/bit.10707
Authors: Michael Fernández, Maria L. Villalonga, Julio Caballero, Alex Fragoso, Roberto Cao, Reynaldo Villalonga
Institutions: University of Matanzas, University of Havana
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W1983545470