Work

Characteristics of the In Vitro Inhibition of Arenavirus Synthesis by Bis-Benzimidazoles

Year: 1974

Type: article

Abstract: The dihydrochloride salt of (S,S)-1,2-bis(5-methoxy-2-benzimidazolyl)-1,2-ethandiol (A37536) inhibits the synthesis of lymphocytic choriomeningitis (LCM), Parana, and Pichinde viruses in L-929 cells. The compound has no direct inactivating effect on LCM virus nor does it affect the adsorption of LCM virus to L cells. The drug-cell interaction is slow. Maximal activity is observed only by exposing cells to the drug at least 8 h prior to LCM virus infection, or by concomitant drug treatment and infection at a low multiplicity. Addition of serum-free media to L cells after LCM virus infection diminishes the activity of A37536. Whereas A37536 exhibits its antiviral activity at concentrations that have little or no effect on L cell division rate, a marked change can be noted in the cell's sensitivity to lysis by standard trypsin dispersal procedures. A37536 has no specific antiviral activity in LCM virus-infected BHK, HeLa, or Vero cells. All of the four tested derivatives of A37536 showed antiviral activity against LCM virus but only at concentrations that reduced the growth rate or were toxic to L cells.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.6.6.747

Authors: J. P. Stella, K. D. Yankaskas, J. H. Morgan, M. P. Fox, C. J. Pfau

Institution: Rensselaer Polytechnic Institute

Language: English

Topic: Viral Infections and Vectors

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases