Work

Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors

Year: 2009

Type: article

Abstract: Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M(1-4) muscarinic receptor subtypes and alpha(2B)-, beta(2)-, and beta(3)-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.

Source: Naunyn-Schmiedeberg s Archives of Pharmacology

DOI: 10.1007/s00210-009-0393-0

Authors: Wisuit Pradidarcheep, Jan Stallen, Wil T. Labruyère, Noshir F. Dabhoiwala, Martin C. Michel, Wouter H. Lamers

Institutions: Srinakharinwirot University, Amsterdam UMC Location University of Amsterdam, University of Amsterdam

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,790