Work

Evaluation of galectin-3 genetic variants and lipid profile in RA patients in North Indian population

Year: 2014

Type: article

Abstract: Rheumatoid arthritis (RA) is a chronic, inflammatory, systemic disease characterized by inflammation and destruction of peripheral joints which leading to deformity and disability. Galectin- 3 is emerging as one of key molecules in pathogenesis of RA. The aim of the present study was to evaluate association of two genetic variants rs4644 and rs4652 of galectin-3 with susceptibility towards RA in North Indian population. The study further involved evaluation of lipid profile variables in cases and controls. The present case-control study involved 200 RA patients diagnosed according to 1987 revised criteria of American college of Rheumatology and 200 unrelated age, sex and ethnicity matched controls. Genomic DNA was isolated from blood samples and genotyping was done with PCR-RFLP. Sample size for genetic association was calculated by CaTS Power calculator (http://www.sph.umich.edu). Serum was analyzed for lipid profile biomarkers using standard reagents and kits. Genotypic distribution of control and RA was compared by odds ratio statistics using medcal software. Differences in lipid profile were analyzed by independent `t’ test using SPSS version 18.0 (IL, USA, and Chicago) The genotypic distribution of +191(A/C) showed significant differences between patients and controls (odds ratio = 1.9552, 95% CI = 1.0461-3.6542, p<0.05). AA genotype was found to be more prevalent in patients in comparison to controls. However, genotypic distribution for +292 (C/A) showed no significant difference between controls and cases (odds ratio = 0.2768, 95% CI = 0.0541-1.4149, p>0.05 ). RA patients were found to be dyslipidemic as indicated by the significantly higher atherogenic index as compared to controls (p<0.01). Galectin-3 may play an important role in pathogenesis of RA.

Source: Molecular Cytogenetics

DOI: 10.1186/1755-8166-7-s1-p66

Authors: Tarnjeet Kaur, Manpreet Kaur, Jatinder Singh, Sukhdev Singh, Sumeet Arora

Institution: Guru Nanak Dev University

Language: English

Topic: Rheumatoid Arthritis Research and Therapies

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,290