Distribution of cellulose-binding proteins among the representative strainsof rumen bacteria.
Year: 1995
Type: article
Abstract: The presence or absence of cellulose-binding proteins (CBPs) in the cell lysate of 31 strains representing 17 species of rumen bacteria was examined.Both carboxymethylcellulose (CMC) and sodium dodecyl sulfate (SDS) were used to elute CBPs of these bacteria from cellulose.For 16 strains classified into 4 species of Fibrobacter succinogenes, Fibrobacter intestinalis, Ruminococcus flavefaciens and Eubacterium cellulosolvens, the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of the CMC-eluted CBPs were identical with those of the SDS-eluted CBPs.For 4 strains representing 4 species of Veillonella parvula, Megasphaera elsdenii, Prevotella ruminicola subsp.ruminicola and Eubacterium ruminantium, the SDS-PAGE patterns of both the CMC-eluted CBPs and the SDS-eluted CBPs were different.In the case of 3 strains representing Ruminococcus bromii, Butyrivibrio fibrisolvens and Ruminobacter amylophilus, CBPs were eluted only with SDS from cellulose, but not with CMC.On the other hand, none of the other strains tested contained a detectable amount of CBPs.Western immunoblot analysis indicated that CBP (120 kDa) of both the 10 strains of F. succinogenes and 1 strain of F intestinalis was antigenically identical with CBP 1 of F succinogenes S85, and CBPs of other bacteria did not cross-react with rabbit antiserum to CBP 1.Many morphological types of microorganisms are associated with plant materials in the rumen microbial ecosystem (1, 3, 6).Under ordinary feeding conditions, microbial populations associated with feed particles are numerically predominant and account for 50--75% of the total microbial population in the rumen (3, 13).In addition, the microbial populations are responsible for the major part of endoglucanase activity, amylase activity and protease activity in the rumen, as
DOI: 10.2323/jgam.41.297
Authors: MAKOTO MITSUMORI, HAJIME MINATO
Institution: National Institute of Animal Health
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W1996646273