Work

Colloidal gold labelling of l,4‐β‐D‐glucan cellobiohydrolase adsorbed on cellulose substrates

Year: 1984

Type: article

Abstract: The 1,4‐β‐D‐glucan cellobiohydrolase I (CBHI) from Trichoderma reesei was labelled with 4–6 nm gold particles. This CBHI‐gold complex still retains 60% of the original CBHI activity and allows good visualization of the enzyme adsorbed at the surface of cellulose mirofibrils or microcrystals. The main features of the adsorption are: rapid and irreversible binding; preference of the crystal edges instead of the crystal surface for the binding; the absence of specificity for the crystal tips where the cellulose chain ends are supposedly located.

Source: FEBS Letters

DOI: 10.1016/0014-5793(84)81124-2

Authors: H. Chanzy, B. Henrissat, R. Vuong

Institutions: Centre National de la Recherche Scientifique, Centre de Recherches sur les Macromolécules Végétales

Language: English

Topic: Biofuel production and bioconversion

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: bronze

OpenAlex ID: W1997146296

FWCI: 3.772

Cites: 12

Cited by: 115

Related to: 10