Work

Characterization of ATPase Activity in Brush Border Membrane Vesicles from the Silkworm, Bombyx mori.

Year: 1991

Type: article

Abstract: Brush border membrane vesicles (BBMV) from the midgut epithelial cells of silkworm larvae were prepared. ATP hydrolyzing activity (ATPase activity) was associated with the BBMV. ATPase activity without Mg2 + was not observed at pH 7 but substantial ATP hydrolyzing activity was observed at pH 7 with Mg2 +. The enzyme required Mn2 +, Mg2 +, or Ca2+ ions. The enzyme also hydrolyzed ITP and GTP but not p-NPP, ADP, or AMP. KNO3 and NEM strongly inhibited the ATPase activity. Behaviours of the ATPase against inhibitors suggested that it resembled vacuolar type ATPase.

Source: Agricultural and Biological Chemistry

DOI: 10.1080/00021369.1991.10871043

Authors: Masayoshi Minami, Leslie S. Indrasith, Hidetaka Hori

Institution: Kubota (Japan)

Language: English

Topic: ATP Synthase and ATPases Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W1998199476

FWCI: 0.334

Cites: 5

Cited by: 10

Related to: 10