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Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLCγ and cooperate to modulate embryonic stem cell survival and proliferation

Year: 2009
Type: article
Abstract: Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.
Source: FEBS Letters
DOI: 10.1016/j.febslet.2009.01.013
Authors: Ya-Li Li, Guang-Zhi Wu, Li Zeng, Gavin S. Dawe, Li Sun, Gabriele Loers, Thomas Tilling, Shu-sen Cui, Melitta Schachner, Zhi-Cheng Xiao
Institutions: National University of Singapore, Singapore General Hospital, Kunming Medical University, Jilin University, Institute of Molecular and Cell Biology, Life Sciences Institute, Universität Hamburg, Rutgers, The State University of New Jersey, GlaxoSmithKline (China)
Language: English
Open Access status: hybrid
Estimated APC paid: $3,980
OpenAlex ID: W1998313143
FWCI: 0.33
Cites: 27
Cited by: 14
Related to: 10