Year: 2010
Type: article
Abstract: Changing environmental conditions have affecting the world’s fauna and flora irreversibly. Objective was genetic characterization anddetermination of phylogenetic relationship of native cattle breeds in Turkey using microsatellite DNA markers as part of a national project titled“In vitro Conservation and Preliminary Molecular Identification of Some Turkish Domestic Animal Genetic Resources-I (TURKHAYGEN-I)”.Blood samples were collected from Anatolian Black (YK), Anatolian Grey (BI), South Anatolian Red (GAK), Native Southern Anatolian Yellow(YGS), East Anatolian Red (DAK) and Zavot (ZAV) cattle. DNA samples were isolated using a standard phenol/chloroform method. A total of20 microsatellite loci were selected from a list suggested by FAO and International Society of Genetics (ISAG). PCR products were separated bycapiller electrophoresis on Beckman Coulter CEQ-8000 Genetic Analysis System, and marker genotypes were determined. In statistical analyses,numbers of alleles, deviation from Hardy-Weingberg Equilibrium parameters were calculated and phylogenetic trees were drawn. A total of 274alleles were observed. The lowest allele number (8) was observed at INRA005 and BM1824 loci and the highest allele number (23) was calculatedfor TGLA122. YGS, YK, GAK and DAK populations were closely localized than BI and ZAV in factorial correspondence analysis. The resultinggenetic parameters and phylogenetic relationship of breeds analyzed are consistent with their today’s geographical locations.
Language: English
Subfield: Genetics
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W19983478