Work

Optimisation of the Formation and Distribution of Protoporphyrin Ix in the Urothelium: an in Vitro Approach

Year: 1999

Type: article

Abstract: PURPOSE: To optimize conditions for photodynamic detection (PDD) and photodynamic therapy (PDT) of bladder carcinoma, urothelial accumulation of protoporphyrin IX (PpIX) and conditions leading to cell photodestruction were studied. MATERIALS AND METHODS: Porcine and human bladder mucosae were superfused with derivatives of 5-aminolevulinic acid (ALA). PpIX accumulation and distribution across the mucosa was studied by microspectrofluorometry. Cell viability and structural integrity were assessed by using vital dyes and microscopy. RESULTS: ALA esters, especially hexyl-ALA, accelerated and regularized urothelial PpIX accumulation and allowed for necrosis upon illumination. CONCLUSIONS: hexyl-ALA used at micromolar concentrations is the most efficient PpIX precursor for PDD and PDT.

Source: The Journal of Urology

DOI: 10.1016/s0022-5347(05)68625-9

Authors: A. MARTI, N. LANGE, H. VAN DEN BERGH, D. SEDMERA, P. JICHLINSKI, P. KUCERA

Institutions: École Polytechnique Fédérale de Lausanne, University of Lausanne, Centre Hospitalier Universitaire Vaudois

Language: English

Topic: Photodynamic Therapy Research Studies

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2000302080

FWCI: 7.345

Cites: 27

Cited by: 94

Related to: 10